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KMID : 1094020180350060273
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Journal of Veterinary Clinics
2018 Volume.35 No. 6 p.273 ~ p.275
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Molecular Detection of Mycoplasma felis Infection in a Cat with Respiratory Symptoms
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Lee Hyun-A
Hong Sun-Hwa
Chung Yung-Ho
Kim Ok-Jin
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Abstract
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A 6-month-old male cat was presented for investigation of depression, loss of appetite, dehydration, paleconjunctival mucous membrane, weight loss, fast heart and respiratory rates, nasal discharge and cough. Nasal swabscollected from the studied cat. As the results of bacterial culture with nasal swabs, it was suspected with Mycoplasmaspp. Also, Mycoplasma species was detected by the PCR reaction with Mycoplasma genus primers. At species PCRassay, the specimens evaluated for the presence of M. felis, M. arginini, M. gateae, and Acholeplasma laidlawii andthe result was visualization of bands from 238 bp in agarose gel 1.5% showing M. felis amplicons in samples. Inconclusion, we detected M. felis in a cat with respiratory disease. PCR was able to detect successfully M. felis infectionin cats.
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KEYWORD
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PCR, Mycoplasma, M. felis, respiratory, cat
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